Molecular Mechanisms of Calcium and Magnesium Binding to Parvalbumin

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Parvalbumin: calcium and magnesium buffering in the distal nephron.

Parvalbumin (PV) is a classical member of the EF-hand protein superfamily that has been described as a Ca(2+) buffer and Ca(2+) transporter/shuttle protein and may also play an additional role in Mg(2+) handling. PV is exclusively expressed in the early part of the distal convoluted tubule in the human and mouse kidneys. Recent studies in Pvalb knockout mice revealed a role of PV in the distal ...

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Kinetic properties of DM-nitrophen binding to calcium and magnesium.

Caged-Ca(2+) compounds such as nitrophenyl-EGTA (NP-EGTA) and DM-nitrophen (DMn) are extremely useful in biological research, but their use in live cells is hampered by cytoplasmic [Mg(2+)]. We determined the properties of Ca(2+) release from NP-EGTA and DMn by using Oregon green BAPTA-5N to measure changes in [Ca(2+)] after ultraviolet flash photolysis in vitro, with or without Mg(2+) present....

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Binding of acrylonitrile to parvalbumin.

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2002

ISSN: 0006-3495

DOI: 10.1016/s0006-3495(02)75472-6